Coding

Part:BBa_K4727008:Design

Designed by: Tommaso Varaschin   Group: iGEM23_Uni-Padua-IT   (2023-09-30)


dCas9 standardized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3378
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To obtain a cassette compatible with the iGEM standards, a PCR mutagenesis was carried out to eliminate the EcoRI site while maintaining the codon usage in E. coli.



Source

The sequence of the dCas9 was obtained from the plasmid pdCas9 (addgene code: 44249).

References